Journal
/
/
Neutrophiles Isolement et analyse pour déterminer leur rôle dans le lymphome cellulaire Sensibilité aux agents thérapeutiques
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
DOI:

14:04 min

March 25, 2016

,

Chapitres

  • 00:05Titre
  • 01:02Primary Leukemic Cell and Neutrophil Isolation
  • 05:25Primary Leukemic Cell and Neutrophil Purity and Co-culture Phenotype Analysis by Flow Cytometry
  • 07:583D Culture Setup and Phenotype Analysis
  • 10:59Results: Analysis of the Role of Neutrophils in Lymphoma Cell Sensitivity to Therapeutic Agents
  • 13:09Conclusion

Summary

Traduction automatique

Neutrophils are the most abundant type of white blood cells. The isolation of neutrophils from human blood by density gradient separation method and the differentiation of human promyelocytic (HL60) cells along the granulocytic pathway are described here; to test their role on sensitivity of lymphoma cells to anti-lymphoma agents.

Vidéos Connexes

Read Article