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Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
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Neurosciences
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Journal JoVE Neurosciences
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

44,166 Views

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10:53 min

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February 26, 2018

DOI:

10.3791/53825-v

DOI:
10.3791/53825-v

•

10:53 min
February 26, 2018

•

53 Views
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1Cell Types Program,Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment,Swedish Neuroscience Institute

Summary

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This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.

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Cite this Article

Ting, J. T., Lee, B. R., Chong, P., Soler-Llavina, G., Cobbs, C., Koch, C., Zeng, H., Lein, E. Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method. J. Vis. Exp. (132), e53825, doi:10.3791/53825 (2018).

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