Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.
Cosette, J., Ben Abdelwahed, R., Donnou-Triffault, S., Sautès-Fridman, C., Flaud, P., Fisson, S. Bioluminescence-Based Tumor Quantification Method for Monitoring Tumor Progression and Treatment Effects in Mouse Lymphoma Models. J. Vis. Exp. (113), e53609, doi:10.3791/53609 (2016).