Here, we present a protocol for the operation and optimization of Active Flow Technology (AFT) column in Curtain flow (CF) mode for enhanced separation performance.
Pravadali-Cekic, S., Kocic, D., Hua, S., Jones, A., Dennis, G., Shalliker, A. Curtain Flow Column: Optimization of Efficiency and Sensitivity. J. Vis. Exp. (112), e53471, doi:10.3791/53471 (2016).