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Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
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Journal JoVE Bio-ingénierie
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy

DOI:
10.3791/53447-v

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11:51 min

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March 01, 2016

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, , , , ,
1Cardiovascular Research Center,Icahn School of Medicine at Mount Sinai, 2The Mindich Child Health and Development Institute,Icahn School of Medicine at Mount Sinai, 3Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine,University of Hong Kong

Chapitres

  • 00:05Titre
  • 00:58Cell Culture and Sorting of Differentiated Cardiomyocytes
  • 04:50Collection of Reaggregated Cardiac Cells
  • 06:32Generation of Human Engineered Cardiac Tissues
  • 09:45Results: Contractile Force Measurement Using Human Engineered Cardiac Tissues
  • 11:05Conclusion

Summary

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Traduction automatique

This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.

Tags

Cardiac Cell TherapyCardiac Contractile FunctionCardiac TherapeuticsHuman Embryonic Stem Cell-derived CardiomyocytesFACSSIRP Alpha-PE/Cy7CD90-FITC

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