Journal
/
Biologie
/
qPCRTag分析 - 高通量，实时定量PCR检测方法的基因分型Sc2.0
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
qPCRTag Analysis – A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
Please note that all translations are automatically generated. Click here for the English version.

qPCRTag分析 - 高通量，实时定量PCR检测方法的基因分型Sc2.0

16,567 Views

•

07:00 min

•

May 25, 2015

DOI:

10.3791/52941-v

DOI:
10.3791/52941-v

•

07:00 min
May 25, 2015

•

43 Views
, , , , ,
1Department of Biochemistry and Molecular Pharmacology,Institute for Systems Genetics, 2Roche Life Science, USA

Summary

Automatically generated

Designer chromosomes of the Synthetic Yeast Genome project, Sc2.0, can be distinguished from their native counterparts using a PCR-based genotyping assay called PCRTagging, which has a presence/absence endpoint. Here we describe a high-throughput real time PCR detection method for PCRTag genotyping.

Explore More Videos

QPCRTag AnalysisHigh ThroughputReal-time PCRSc2.0 GenotypingSynthetic Yeast GenomePCRTagOpen Reading FrameORFSynthetic ChromosomeWild Type ChromosomeGenome EngineeringMultiplex PCR

Read Article

Cite this Article

Mitchell, L. A., Phillips, N. A., Lafont, A., Martin, J. A., Cutting, R., Boeke, J. D. qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping. J. Vis. Exp. (99), e52941, doi:10.3791/52941 (2015).

Download .ris file

Copy

Share Video

.

Copy