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Enriquecimiento de la célula automatizada de citomegalovirus células T específicas para aplicaciones clínicas utilizando el Sistema de citoquinas de captura
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Enriquecimiento de la célula automatizada de citomegalovirus células T específicas para aplicaciones clínicas utilizando el Sistema de citoquinas de captura
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10:24 min
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October 05, 2015
DOI:
Summary
Automatically generated
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
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Cite this Article
Kumaresan, P., Figliola, M., Moyes, J. S., Huls, M. H., Tewari, P., Shpall, E. J., Champlin, R., Cooper, L. J. Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System. J. Vis. Exp. (104), e52808, doi:10.3791/52808 (2015).
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