/
/
Arricchimento cellulare automatizzata di Citomegalovirus specifici linfociti T per le applicazioni cliniche che utilizzano il sistema di citochine cattura
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Arricchimento cellulare automatizzata di Citomegalovirus specifici linfociti T per le applicazioni cliniche che utilizzano il sistema di citochine cattura
12,238 Views
•
10:24 min
•
October 05, 2015
DOI:
Summary
Automatically generated
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
Explore More Videos
Automated Cell EnrichmentCytomegalovirus-specific T CellsClinical ApplicationsCytokine-capture SystemAllogeneic Hematopoietic Stem-Cell TransplantationViral-specific T CellsGamma-interferonCytokine Capture System (CCS)CliniMACS ProdigyMagnetic Activated Cell SortingCMV Pp65-specific T CellsAutomated Cell Enrichment DeviceGMP FacilitiesBlood Banking Facilities
Read Article
Cite this Article
Kumaresan, P., Figliola, M., Moyes, J. S., Huls, M. H., Tewari, P., Shpall, E. J., Champlin, R., Cooper, L. J. Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System. J. Vis. Exp. (104), e52808, doi:10.3791/52808 (2015).
Copy