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Caracterização de Thymic Settling Progenitores no embrião do rato Usando<em> In Vivo</em> E<em> In Vitro</em> Os ensaios
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
DOI:

08:56 min

June 09, 2015

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Chapitres

  • 00:05Titre
  • 01:51Embryo Dissection
  • 02:52Thymus Isolation
  • 03:49E14 Thymic Lobe Progenitor Colonization: Hanging Drop Technique
  • 04:53Grafting Under the Kidney Capsule
  • 06:22Results: Representative Thymic Settling Progenitor (TSP) Propagation
  • 08:07Conclusion

Summary

Traduction automatique

This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.

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