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Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
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June 09, 2015
DOI:
1Research Center Growth and Signaling, INSERM U845,Institut Cochin, 2Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy,University of Pierre & Marie Curie, 3Unit for Lymphopoiesis, Immunology Department, INSERM U668,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur
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This article describes in vivo and in vitro methodology to characterize the thymic settling progenitors by the analysis of the kinetics of generation, phenotype and numbers of their T cell progeny.
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Ramond, C., Bandeira, A., Berthault, C., Pereira, P., Cumano, A., Burlen-Defranoux, O. Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays. J. Vis. Exp. (100), e52795, doi:10.3791/52795 (2015).
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