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Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
Journal JoVE
Biologie
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Journal JoVE Biologie
Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
DOI:

09:18 min

May 03, 2015

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Chapitres

  • 00:05Titre
  • 01:29Ion-selective Self-referencing Microelectrode Calibration
  • 04:16Ion Flux Measurements
  • 07:38Results: Evidence of Potassium Efflux During Single Cell Wound-healing
  • 08:46Conclusion

Summary

Traduction automatique

Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.

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