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An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
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Environnement
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Journal JoVE Environnement
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates

DOI:
10.3791/52781-v

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13:24 min

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April 09, 2015

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, , , , , , , , ,
1Biology Department,University of the Sciences, 2Biology Department,Arcadia University, 3Biology Department,Immaculata University, 4Biology/Clinical Laboratory Science,Neumann University, 5Science Department,Cabrini College

Chapitres

  • 00:05Titre
  • 01:39Preparation of Arthrobacter Cells for Phage Isolation
  • 02:36Collection of Phage from Soil Samples
  • 04:38Phage Isolation
  • 06:45Phage Purification
  • 11:20Results: Unique Arthrobacter Bacteriophage Species are Isolated by the Enrichment Technique
  • 12:54Conclusion

Summary

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Traduction automatique

We present an enrichment protocol for the isolation of bacteriophages infecting bacteria in the Arthrobacter genus. This enrichment protocol produces fast and reproducible results for the isolation and amplification of Arthrobacter phages from soil isolates.

Tags

ArthrobacterBacteriophageEnrichment TechniqueSoil SampleIsolationIndicator BacteriaArthrobacter Sp. KY3901Phage Diversity

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