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Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals
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Biologie du développement
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Journal JoVE Biologie du développement
Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

Assessment of Endothelial Cell Migration After Exposure to Toxic Chemicals

DOI:
10.3791/52768-v

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07:07 min

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July 10, 2015

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, , , , , ,
1Bundeswehr Institute of Pharmacology and Toxicology, 2Walther Straub Institute of Pharmacology and Toxicology,Ludwig-Maximilians-Universität München, 3Department of Molecular and Cellular Sports Medicine,German Sports University Cologne

Chapitres

  • 00:05Titre
  • 01:15Early Endothelial Cell Culture
  • 02:03Boyden Chamber
  • 03:28Wound Healing Assay, Cell Tracking, and Mitochondrial Membrane Potential Assessment
  • 05:17Results: Endothelial Cell Migration After Exposure to Toxic Chemicals
  • 06:37Conclusion

Summary

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Traduction automatique

Investigation of early endothelial cell (EEC) migration is important to understand the pathophysiology of certain illnesses and to potentially identify novel strategies for therapeutic intervention. The following protocol describes techniques to assess cell migration that have been adapted for the investigation of EEC.

Tags

Keywords: Endothelial Cell MigrationToxic ChemicalsWound HealingChemical Warfare AgentsSulfur MustardNitrogen MustardVasculogenesisAngiogenesisBoyden ChamberWound Healing AssayTrack AnalysisTMRMMitochondrial Membrane Potential

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