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Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

9,985 Views

09:07 min

May 27, 2015

DOI:

09:07 min
May 27, 2015

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Summary

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We describe a method for the generation of in vitro derived mast cells, their engraftment into mast cell-deficient mice, and the analysis of the phenotype, numbers and distribution of engrafted mast cells at different anatomical sites. This protocol can be used to assess the functions of mast cells in vivo.

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