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Immunology and Infection

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JoVE Journal Immunology and Infection
High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
 

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Article DOI: 10.3791/52650-v • 10:00 min • March 24th, 2015
March 24th, 2015 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 1
1Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Center for Drug Evaluation and Research, US Food and Drug Administration

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This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.

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High-throughput Quantitative Real-time RT-PCR Assay Expression Profiles Types I And III Interferon Subtypes QRT-PCR Assay Analysis Human IFN Subtypes 384-well Plate Format Locked Nucleic Acid (LNA) Molecular Beacon (MB) Probes Standards Automated Multichannel Pipetting Plate Drying Type I Interferons (IFN) IFN-α Subtypes Sequence Identity Type III IFN IFN-β2 IFN-β3 Reliable Reproducible Inexpensive Means
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