/
/
Manipülasyon ve<em> İn Vitro</emVe> Olgunlaşma<em> Xenopus laevis</emIntrasitoplazmik sperm enjeksiyonu izledi> oositler, Embriyonik Geliştirme Çalışması
This content is Free Access.
Manipülasyon ve<em> İn Vitro</emVe> Olgunlaşma<em> Xenopus laevis</emIntrasitoplazmik sperm enjeksiyonu izledi> oositler, Embriyonik Geliştirme Çalışması
21,766 Views
•
09:22 min
•
February 09, 2015
DOI:
Summary
Automatically generated
We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.
Read Article
Cite this Article
Miyamoto, K., Simpson, D., Gurdon, J. B. Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development. J. Vis. Exp. (96), e52496, doi:10.3791/52496 (2015).
Copy