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Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
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Immunologie et infection
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Journal JoVE Immunologie et infection
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

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08:27 min

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March 24, 2015

DOI:

10.3791/52159-v

DOI:
10.3791/52159-v

•

08:27 min
March 24, 2015

•

1 Views
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1Experimmune, A Center for Immunotherapeutic Development, Department of Immunotherapeutics and Biotechnology,Texas Tech University Health Sciences Center, 2Resonant Sensors Incorporated, 3University of Texas Arlington

Summary

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Intact class I HLA/peptide complexes are shed by cancer cells, representing a potential relevant cancer biomarker. Utilizing label-free sensor technology and T-cell receptor mimicking monoclonal antibodies, detection of shed MIF/HLA-A*02:01 complexes in MDA-MB-231 cell supernatants, spiked human serum, and patient plasma is demonstrated, enabling development of a novel cancer diagnostic platform.

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Breast CancerHuman Leukocyte Antigen (HLA)T-cell Receptor Mimic (TCRm) Monoclonal AntibodiesMacrophage Migration Inhibitory Factor (MIF)NY-ESO-1Label-free BiosensorGuided-mode Resonance (GMR) SensorCancer BiomarkersPersonalized MedicineCompanion DiagnosticsImmunotherapy

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Weidanz, J. A., Doll, K. L., Mohana-Sundaram, S., Wichner, T., Lowe, D. B., Gimlin, S., Wawro Weidanz, D., Magnusson, R., Hawkins, O. E. Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology. J. Vis. Exp. (97), e52159, doi:10.3791/52159 (2015).

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