Journal
/
Immunologie et infection
/
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

DOI:
10.3791/51894-v

•

06:40 min

•

September 09, 2014

•

, , ,
1Department of Pathology,Northwestern University

Chapitres

  • 00:05Titre
  • 01:21Purification of Fetal Liver Erythroblasts
  • 02:32Purification of TER119 Negative Erythroblasts
  • 03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
  • 04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
  • 06:08Conclusion

Summary

Traduction automatique

Traduction automatique

We present an in vitro mouse fetal liver erythroblast culture system that dissects the early and late stages of terminal erythropoiesis. This system facilitates functional analysis of specific genes in different developmental stages.

Tags

Mouse Fetal LiverErythropoiesisErythroid Burst Forming Units (BFU-E)Erythroid Colony Forming Units (CFU-E)Terminal ErythroblastsTER119Erythroid Progenitor CellsGene Function AnalysisEarly And Late Stages Of Terminal ErythropoiesisCDNA OverexpressionShRNA KnockdownErythropoietin (Epo)

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Introducing Shear Stress in the Study of Bacterial Adhesion

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Read Article