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Journal / Bioengineering / 管式膜网络在心肌细胞的心房和心室分析…
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Bioengineering

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JoVE Journal Bioengineering
管式膜网络在心肌细胞的心房和心室分析
 
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管式膜网络在心肌细胞的心房和心室分析

Article DOI: 10.3791/51823-v • 10:30 min • October 15th, 2014
October 15th, 2014 •
*1,2,3, *1,2, 1,2, 1,2,3,4
1Heart Research Center Goettingen, 2Clinic of Cardiology & Pulmonology, University Medical Center Goettingen, 3German Center for Cardiovascular Research (DZHK) partner site Goettingen, 4BioMET, Center for Biomedical Engineering & Technology, University of Maryland School of Medicine
* These authors contributed equally

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在心肌细胞，管式膜结构形成细胞内的网络。我们描述了优化方案从小鼠心脏肌细胞I）的隔离，包括质量控制，二）活细胞染色国家的最先进的荧光显微镜，及iii）直接图像分析来量化成分的复杂性和膜细胞的可塑性网络。

Tags

生物工程学杂志，第92期，心肌细胞，心房，心室，心脏，原代细胞分离，荧光显微镜，膜小管，横向轴向细管系统，图像分析，图像处理，T细管，胶原酶
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