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A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
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A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Chapitres
- 00:05Titre
- 01:33Create Reference Image, Aligning Tissue for Dissected, and Annotate Slide
- 03:29Load Consumable Mill Bit
- 04:20Dissecting Tissue
- 04:59Emptying and Removing Consumable Mill Bit
- 05:32Results: Granulomas Caused by Mycobacterium tuberculosis can be Microdissected for RNA Extraction
- 06:58Conclusion
Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.
