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在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪
Journal JoVE
Biologie
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Journal JoVE Biologie
In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow
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在造血干细胞和祖细胞的成年小鼠颅骨骨髓体内四维跟踪

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12:54 min

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September 04, 2014

DOI:

10.3791/51683-v

DOI:
10.3791/51683-v

•

12:54 min
September 04, 2014

•

3 Views
, ,
1Department of Life Science and Facility for Imaging by Light Microscopy,Imperial College London, 2Department of Life Sciences,Imperial College London

Summary

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造血干细胞和祖细胞（HSPCs）和骨髓龛之间的相互作用的性质知之甚少。定制的硬件修改和多步获取协议允许使用双光子共焦显微术图像体外骨髓区域内驻留标记HSPCs，跟踪相互作用和运动。

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Hematopoietic Stem CellsHSCsHematopoietic Stem And Progenitor CellsHSPCsBone Marrow MicroenvironmentIn Vivo Imaging4-dimensional TrackingConfocal ImagingTwo-photon ImagingOsteoblastic Cells

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Cite this Article

Scott, M. K., Akinduro, O., Lo Celso, C. In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow. J. Vis. Exp. (91), e51683, doi:10.3791/51683 (2014).

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