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原位检测自身反应性CD4 + T细胞在大脑和心脏使用主要组织相容性复合II类Dextramers的
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August 01, 2014
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通过直接染色与主要组织相容性复合物Ⅱ类dextramers检测自身反应性CD4 + T细胞在大脑和心脏的协议已经本报告中进行了描述。为综合分析，一种可靠的方法来枚举抗原特异性CD4 + T细胞在原位的频率也被设计出来。
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Massilamany, C., Gangaplara, A., Jia, T., Elowsky, C., Li, Q., Zhou, Y., Reddy, J. In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers. J. Vis. Exp. (90), e51679, doi:10.3791/51679 (2014).
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