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Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time
 

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Article DOI: 10.3791/51655-v • 14:25 min • May 20th, 2014
May 20th, 2014 •
1, 1,2
1Reproductive Development and Cancer Laboratory, MIMR-PHI Institute of Medical Research, 2Department of Developmental Biology and Anatomy, Monash University

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Ovarian cancer cell invasion into the mesothelial lining of the peritoneum is a dynamic process over time. Utilizing a real time analyzer, the invasive capacity of ovarian cancer cells in a spheroid-mesothelial cell co-culture model can be quantified over prolonged time periods, providing insights into factors regulating the metastatic process.

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Ovarian Cancer Spheroid Attachment Invasion Mesothelial Cells Real-time Assessment Metastasis Peritoneal Fluid Multicellular Structures Secondary Tumors Nonadherent Environment Spheroid-mesothelial Co-culture Model Invasive Capacity Cell Analyzer Quantitative Measurements Endpoint Assays Real-time Microscopy Image Analyses
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