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Anvendelse af fluorescerende Skud Arrays for Vurdering af T-celle respons<em> In vivo</em
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Immunologie et infection
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Journal JoVE Immunologie et infection
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Anvendelse af fluorescerende Skud Arrays for Vurdering af T-celle respons<em> In vivo</em

DOI:
10.3791/51627-v

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12:09 min

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June 19, 2014

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, , ,
1Department of Immunology, John Curtin School of Medical Research,Australian National University

Chapitres

  • 00:05Titre
  • 01:39Fluorescent Target Array Preparation: CTV and CFSE Labeling
  • 04:54FTA Preparation: Peptide Pulsing and Cell Washing
  • 07:50FTA Preparation: CPD Cell Labeling
  • 09:43FTA Assay in Response to VV/HIV Epitopes
  • 11:45Conclusion

Summary

Traduction automatique

Traduction automatique

Muligheden for at overvåge T-cellereaktioner i detaljer in vivo er vigtig for udviklingen af vores forståelse af immunresponset. Her beskriver vi brug af fluorescerende target arrays (FTA) i en in vivo T-celle analyse, der vurderer> 250 parametre samtidigt ved flowcytometri.

Tags

Fluorescent Target ArrayFTACFSECTVCPDMHC Class IMHC Class IICD8+ T CellsCD4+ T CellsT Cell ResponseIn VivoFlow CytometryAntigen EpitopesRecombinant Pox Virus Vaccine

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