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Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Journal JoVE Immunologie et infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

18,293 Views

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12:55 min

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January 17, 2015

DOI:

10.3791/51492-v

DOI:
10.3791/51492-v

•

12:55 min
January 17, 2015

•

4 Views
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1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research,University of Toronto, 3Antibiome Center,University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology,The University of Chicago

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Phage DisplaySynthetic Antibody LibrariesHigh-throughput SelectionFab FragmentsAntigen CharacterizationAntibody DiscoveryImmunoassays

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Miersch, S., Li, Z., Hanna, R., McLaughlin, M. E., Hornsby, M., Matsuguchi, T., Paduch, M., Sääf, A., Wells, J., Koide, S., Kossiakoff, A., Sidhu, S. S. Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries. J. Vis. Exp. (95), e51492, doi:10.3791/51492 (2015).

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