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Human Neutrophil Flow Chamber Adhesion Assay
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July 02, 2014
DOI:
1Genetics and Genomic Sciences Graduate Program,University of Alabama at Birmingham, 2Birmingham Veterans Affairs Medical Center, 3Department of Pathology,University of Alabama at Birmingham, 4Department of Biomedical Engineering,University of Alabama at Birmingham, 5Department of Medicine,University of Alabama at Birmingham
Summary
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A method of quantitating neutrophil adhesion is reported. This method creates a dynamic flow environment similar to that encountered in a blood vessel. It allows the investigation of neutrophil adhesion to either purified adhesion molecules (ligand) or endothelial cell substrate (HUVEC) in a context similar to the in vivo environment with sheer stress.
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Zhou, Y., Kucik, D. F., Szalai, A. J., Edberg, J. C. Human Neutrophil Flow Chamber Adhesion Assay. J. Vis. Exp. (89), e51410, doi:10.3791/51410 (2014).
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