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Immunology and Infection

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JoVE Journal Immunology and Infection
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
 

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Article DOI: 10.3791/51256-v • 07:35 min • April 25th, 2014
April 25th, 2014 •
1, 1, 1, 1, 1, 1, 1, 1
1Akonni Biosystems, Inc.

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An amplification microarray combines asymmetric PCR amplification and microarray hybridization into a single chamber, which significantly streamlines microarray workflow for the end user. Simplifying microarray workflow is a necessary first step for creating microarray-based diagnostics that can be routinely used in lower-resource environments.

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Multi-drug Resistant Mycobacterium Tuberculosis Amplification Microarray MDR-TB Microarray Workflow Lower-resource Environments Asymmetric PCR Amplification Target Size Selection Target Labeling Microarray Hybridization Batch Processing Method Genotyping Low-density Gel Element Microarray On-chip Wash Steps Amplicon Method And System Multiplexing Primer Pairs Sensitivity And Specificity Performance Metrics Probes Immobilized On The Array
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