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December 17, 2013
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This protocol describes a sensitive, cell-based cytotoxicity assay. By enumerating the decrease in frequency of live target CD4+ T cells in the presence of an increasing number of effector CD8+ T cells, this assay allows for the direct assessment of cytolytic activity of antigen-specific CD8+ T cells.
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Noto, A., Ngauv, P., Trautmann, L. Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity. J. Vis. Exp. (82), e51105, doi:10.3791/51105 (2013).
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