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Immunology and Infection

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JoVE Journal Immunology and Infection
Cecal hemorroider og punkterings indusert Sepsis som en modell for å studere Autophagy i Mus
 
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Cecal hemorroider og punkterings indusert Sepsis som en modell for å studere Autophagy i Mus

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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Eksperimentell sepsis kan bli indusert i mus ved hjelp av cecal ligering og punktering (CLP)-metoden. Gjeldende forskrifter for å bestemme autophagy in vivo i forbindelse med CLP-indusert sepsis er presentert her: En protokoll for å måle ved hjelp av autophagy (GFP)-LC3 mus, og en protokoll for å måle autophagosome dannelse ved elektronmikroskopi.

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Infeksjon autophagosome Autophagy cecal ligation og punktering mus sepsis
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