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Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices
Journal JoVE
Médecine
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Journal JoVE Médecine
Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

DOI:
10.3791/50970-v

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13:32 min

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January 14, 2014

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, , ,
1Institute of Anatomy and Cell Biology,Justus-Liebig-University

Chapitres

  • 00:05Titre
  • 01:44Isolation of Murine Lungs and Preparation of Precision Cut Lung Slices
  • 06:05Videomorphometric Analysis of Intrapulmonary Arteries of Precision Cut Lung Slices
  • 08:50Analysis of Vasoreactivity and Graphic Presentation
  • 09:30Results: Hypoxia-induced Vasoconstriction of Pre-acinar and Intra-acinar Arteries
  • 12:42Conclusion

Summary

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Traduction automatique

Hypoxic pulmonary vasoconstriction (HPV) is an important physiological phenomenon by which at alveolar hypoxia lung perfusion is matched to ventilation. The major vascular segment contributing to HPV is the intra-acinar artery. Here, we describe our protocol for the analysis of HPV of murine pulmonary vessels with diameters of 20-100 μm.

Tags

Keywords: Videomorphometric AnalysisHypoxic Pulmonary VasoconstrictionIntra-pulmonary ArteriesMurine Precision Cut Lung SlicesIntra-acinar ArteriesPre-acinar ArteriesVasoreactivityAlveolar HypoxiaVon Euler-Liljestrand MechanismPulmonary PerfusionVentilation Matching

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