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Rapid Generation of Amyloid from Native Proteins In vitro
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Journal JoVE Biologie
Rapid Generation of Amyloid from Native Proteins In vitro

Rapid Generation of Amyloid from Native Proteins In vitro

DOI:
10.3791/50869-v

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05:48 min

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December 05, 2013

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, ,
1Department of Immunology,The University of Texas MD Anderson Cancer Center

Chapitres

  • 00:05Titre
  • 01:11Prepare Protein-only Amyloid Directly from Native Proteins
  • 02:06Prepare Hybrid Amyloid from Stabilized Soluble Oligomers of Native Proteins
  • 04:01Results: Analysis of the Amyloid and Soluble Protein Oligomer
  • 05:25Conclusion

Summary

Traduction automatique

Traduction automatique

Proteins can either adopt a native structure or misfold into insoluble amyloid. Conditions that favor the misfolding pathway lead to the formation of different types of amyloid fibrils. The methods described here allow rapid conversion of native proteins into amyloid in vitro.

Tags

AmyloidProtein MisfoldingProtein AggregationAmyloid FibrilsBeta-sheet Rich StructureIn Vitro Amyloid GenerationSoluble Amyloid PrecursorNonproteinaceous Amyloid ComponentsNucleic AcidsGlycosaminoglycans

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