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Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
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Immunologie et infection
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Journal JoVE Immunologie et infection
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

DOI:
10.3791/50677-v

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11:22 min

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August 23, 2013

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, ,
1Department of Anesthesia and Perioperative Care,University of California, San Francisco, 2Graduate Program in Biomedical Sciences,University of California, San Francisco

Chapitres

  • 00:05Titre
  • 01:48Plating and Maintenance of Human Microvascular Endothelial Cells (HMVEC)
  • 02:33Neutrophil Isolation using Polymorphprep
  • 04:31Neutrophil Labeling with Calcein AM
  • 05:47Neutrophil Binding Assay
  • 07:43Results: Measuring Neutrophil Adhesion to Activated Primary HMVEC under Static Conditions
  • 10:57Conclusion

Summary

Traduction automatique

Traduction automatique

Neutrophil adherence to the activated endothelium at sites of infection is an integral component of the host's inflammatory response. Described in this report is a neutrophil binding assay that allows for the in vitro quantitation of primary human neutrophil binding to endothelial cells activated by inflammatory mediators under static conditions.

Tags

Neutrophil AdhesionEndothelial CellsInflammationIn Vitro AssayFluorescence MicroscopyCalcein AMQuantitative Analysis

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