/
/
Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
This content is Free Access.
Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
21,302 Views
•
08:35 min
•
October 01, 2013
DOI:
Summary
Automatically generated
We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.
Read Article
Cite this Article
Jiang, H., Barbier, H., Brutnell, T. Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses. J. Vis. Exp. (80), e50527, doi:10.3791/50527 (2013).
Copy