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07:07 min
July 04, 2013
DOI:
10.3791/50513-v
在这里,我们描述了一个协议,光遗传学操纵运动元活动,同时监控电机输出的变化(肌肉收缩)半完好<em>果蝇</em>幼虫使用在传统的激光共聚焦显微镜。这项技术使研究人员能够实现的神经活动几neuromeres在局部扰动阐明电机电路的动态。
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Matsunaga, T., Fushiki, A., Nose, A., Kohsaka, H. Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion. J. Vis. Exp. (77), e50513, doi:10.3791/50513 (2013).
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