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光遗传学的扰动激光照射半完好的神经活动<em>果蝇</em>幼虫运动

Journal JoVE
Neurosciences
Author Produced

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Journal JoVE Neurosciences

Optogenetic Perturbation of Neural Activity with Laser Illumination in Semi-intact Drosophila Larvae in Motion

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光遗传学的扰动激光照射半完好的神经活动<em>果蝇</em>幼虫运动

DOI:

10.3791/50513-v

•

07:07 min

•

July 04, 2013

•

Teruyuki Matsunaga, Akira Fushiki, Akinao Nose², Hiroshi Kohsaka

¹Department of Complexity Science and Engineering, Graduate School of Frontier Sciences,The University of Tokyo, ²Department of Physics, Graduate School of Science,The University of Tokyo

Chapitres

00:01Titre
00:53Introduction
01:41Larvae preparation
02:46Microscope setup
03:02Dissection
04:21Imaging with laser illumination
05:26Representative results
06:25Conclusion

Summary

Traduction automatique

在这里，我们描述了一个协议，光遗传学操纵运动元活动，同时监控电机输出的变化（肌肉收缩）半完好<em>果蝇</em>幼虫使用在传统的激光共聚焦显微镜。这项技术使研究人员能够实现的神经活动几neuromeres在局部扰动阐明电机电路的动态。

Tags

Keywords: Optogenetics Drosophila Larvae Locomotion Neural Activity Laser Illumination Channelrhodopsin Halorhodopsin Ventral Nerve Cord Sub-genetic Resolution

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