Journal
/
Immunologie et infection
/
Isolamento de precursor de células B subconjuntos de Sangue do Cordão Umbilical
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood

Isolamento de precursor de células B subconjuntos de Sangue do Cordão Umbilical

17,582 Views

•

14:06 min

•

April 16, 2013

DOI:

10.3791/50402-v

DOI:
10.3791/50402-v

•

14:06 min
April 16, 2013

•

3 Views
, , , ,
1Department of Pathology and Anatomical Sciences,University of Missouri-Columbia, 2Laboratory for Infectious Disease Research,University of Missouri-Columbia

Summary

Automatically generated

Aqui descrevemos um protocolo para o isolamento de subconjuntos de precursor células B do sangue do cordão umbilical. A quantidade e qualidade suficientes de ácidos nucleicos podem ser extraídos a partir das células e usadas em ensaios subsequentes utilizando DNA ou RNA.

Explore More Videos

Umbilical Cord BloodHematopoietic Progenitor CellsPrecursor B-cellsB-cell DifferentiationCell IsolationCell SortingCell Surface MarkersMononuclear CellsBiotinFluorescent LabelingDownstream Nucleic Acid Assays

Read Article

Cite this Article

Almamun, M., Schnabel, J. L., Gater, S. T., Ning, J., Taylor, K. H. Isolation of Precursor B-cell Subsets from Umbilical Cord Blood. J. Vis. Exp. (74), e50402, doi:10.3791/50402 (2013).

Download .ris file

Copy

Share Video

.

Copy