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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
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Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato
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January 31, 2013
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Optogenetic techniques have made it possible to study the contribution of specific neurons to behavior. We describe a method in larval zebrafish for activating single somatosensory neurons expressing a channelrhodopsin variant (ChEF) with a diode-pumped solid state (DPSS) laser and recording the elicited behaviors with a high-speed video camera.
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Palanca, A. M. S., Sagasti, A. Optogenetic Activation of Zebrafish Somatosensory Neurons using ChEF-tdTomato. J. Vis. Exp. (71), e50184, doi:10.3791/50184 (2013).
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