/
/
Stereotactic Injection of MicroRNA-expressing Lentiviruses to the Mouse Hippocampus CA1 Region and Assessment of the Behavioral Outcome
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:37Lentivirus Preparation
- 03:04Preparing Animals for Surgery
- 03:46Drilling the Skull
- 05:26Injection of the Lentivirus
- 06:33Wound Sealing and Recovery
- 07:11miR Injection into the Hippocampus and Behavioral Outcomes
- 08:12Conclusion
MicroRNAs have significant roles in brain structure and function. Here we describe a method to enforce hippocampal miRNA over-expression using stereotactic injection of an engineered miRNA-expressing lentivirus. This approach can serve as a relatively rapid way to assess the in vivo effects of over-expressed miRNAs in specific brain regions.
