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Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe
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Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe
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February 24, 2013
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Summary
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Olfactory cues mediate many different behaviors in insects, and are often complex mixtures comprised of tens to hundreds of volatile compounds. Using gas chromatography with multi-channel recording in the insect antennal lobe, we describe a method for the identification of bioactive compounds.
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IdentificationOlfactory VolatilesGas Chromatography-multi-unit RecordingsGCMRInsect Antennal LobeSensory StimuliBehavioral ResponsePhysiological ResponseOlfactionInsectsOdor StimuliReproductionHabitat SelectionChemical SensingAgricultureHuman HealthPollinationHerbivoryTransmission Of DiseaseEcological ProcessesHuman Food ResourcesVolatile Identification TechniquesGas Chromatography-coupled Electroantennogram Recording (GC-EAG)Gas Chromatography-coupled Single Sensillum Recordings (GC-SSR)Bioactive CompoundsMulti-channel Electrophysiological Recordings
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Byers, K. J. R. P., Sanders, E., Riffell, J. A. Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings (GCMR) in the Insect Antennal Lobe. J. Vis. Exp. (72), e4381, doi:10.3791/4381 (2013).
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