Journal
/
Biologie
/
Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

DOI:
10.3791/4373-v

•

11:22 min

•

February 13, 2013

•

, ,
1Department of Science,Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology,Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center,Queens College, The City University of New York (CUNY)

Chapitres

  • 00:05Titre
  • 01:20Preparing RNAi Feeding Plates Carrying Target Gene Sequence
  • 03:14Culture Worms on the RNAi Feeding Plates
  • 04:58Score Body Size Phenotypes of RNAi Treated Worms
  • 08:46Body Size Regulation by the DBL-1/TGF-β Pathway
  • 11:00Conclusion

Summary

Traduction automatique

Traduction automatique

We demonstrate how to use the RNAi feeding technique to knock down target genes and score body size phenotype in C. elegans. This method could be used for a large scale screen to identify potential genetic components of interest, such as those involved in body size regulation by DBL-1/TGF-β signaling.

Tags

RNA-mediated InterferenceScreening GenesBody Size RegulationNematode C. ElegansDouble-strand RNAKnockdown Target Gene ExpressionRNAi VectorRNAi OligosMicroinjectionFeeding StrategyBody Size PhenotypeTGF-β Signaling ComponentsRNAi Hypersensitive Strains

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Application of a C. elegans Dopamine Neuron Degeneration Assay for the Validation of Potential Parkinson’s Disease Genes

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Antibody Staining in C. Elegans Using "Freeze-Cracking"

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Methods to Assess Subcellular Compartments of Muscle in C. elegans

Measuring Glutathione-induced Feeding Response in Hydra

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Measuring RAN Peptide Toxicity in C. elegans

Read Article