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Quantitative Measurement of the Immune Response and Sleep in Drosophila
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Quantitative Measurement of the Immune Response and Sleep in Drosophila
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December 04, 2012
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To understand a link between the immune response and behavior, we describe a method to measure locomotor behavior in Drosophila during bacterial infection as well as the ability of flies to mount an immune response by monitoring survival, bacterial load, and real-time activity of a key regulator of innate immunity, NFκB.
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Quantitative MeasurementImmune ResponseSleepDrosophilaInfectionExcess SleepMammalsDrosophila MelanogasterRobust Immune SystemMultidisciplinary ApproachLocomotor BehaviorPathogenic Strain Of BacteriaDuration Of SurvivalImmune FunctionNFκBTranscription FactorInnate Immune ResponseBacterial ClearanceResistance To InfectionTolerance To Infection
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Kuo, T., Handa, A., Williams, J. A. Quantitative Measurement of the Immune Response and Sleep in Drosophila. J. Vis. Exp. (70), e4355, doi:10.3791/4355 (2012).
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