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Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
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Neurosciences
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Journal JoVE Neurosciences
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

DOI:
10.3791/4281-v

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12:43 min

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October 17, 2012

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, , , , , , ,
1Natural Science Division,Pepperdine University

Chapitres

  • 00:05Titre
  • 01:52Pre-experimental Preparations
  • 02:47Isolation of Auditory and Vestibular Labyrinth
  • 05:28Hair Cell Isolation
  • 07:18Patch Clamping Zebrafish Hair Cells
  • 10:12Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
  • 12:04Conclusion

Summary

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The purpose of this video is to demonstrate procedures for obtaining healthy, intact hair cells from the inner ear organs of adult zebrafish and then using them for patch clamp studies aimed at characterizing the biophysical properties of their voltage-gated channels.

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Patch Clamp RecordingsInner Ear Hair CellsZebrafishVoltage-gated ChannelsSensory Hair CellsIsolation MethodHair Cell SizeHair Cell MorphologyDiversityPatch Clamp RecordingsZebrafish Model SystemMutantsHearing And BalanceTransgenic LinesGenetic AnalysisElectrophysiological MethodsSensory Modalities

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