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Immunologie et infection

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耐高温溶菌酶的定向进化的一个新的筛选方法

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

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耐高温溶菌酶的定向进化的一个新的筛选方法

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13:30 min

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November 07, 2012

DOI:

DOI:

•

13:30 min

November 07, 2012

•

1 Views

Ryan D. Heselpoth¹, Daniel C. Nelson¹

¹Institute for Bioscience and Biotechnology Research,University of Maryland

Summary

热稳定性工程领域的一种新型的定向进化方法的具体开发，从而验证溶菌酵素。随机诱变只有一轮中，演进式溶菌酶，PlyC 29C3，显示后大于两倍的残留活性相比升高的温度下温育后的野生型蛋白。

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