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小规模的核提取物，基因表达机具功能分析
Journal JoVE
Biologie
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Journal JoVE Biologie
Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
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小规模的核提取物，基因表达机具功能分析

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08:05 min

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June 27, 2012

DOI:

10.3791/4140-v

DOI:
10.3791/4140-v

•

08:05 min
June 27, 2012

•

1 Views
, , ,
1Department of Cell Biology,Harvard Medical School

Summary

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一个健壮的，小规模的HeLa细胞的核提取物制备的协议。该协议是为需要使用小种群的细胞，如细胞治疗药物或RNAi，有价值的实验。该方法应该适用于各种各样的基因表达分析和其他类型的细胞，包括病人的细胞。

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Cite this Article

Folco, E. G., Lei, H., Hsu, J. L., Reed, R. Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries. J. Vis. Exp. (64), e4140, doi:10.3791/4140 (2012).

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