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Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
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June 27, 2012
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A protocol for preparation of robust, small-scale HeLa nuclear extracts is described. This protocol is valuable for assays that require use of small populations of cells, such as cells treated with drugs or RNAi. The method should be applicable to a wide variety of gene expression assays and other cell types, including patient cells.
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Folco, E. G., Lei, H., Hsu, J. L., Reed, R. Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries. J. Vis. Exp. (64), e4140, doi:10.3791/4140 (2012).
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