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Langerhans İzolasyonu Fare Islet Saflaştırılmış Collagenase ve Nötr Proteaz bir kombinasyonu kullanılarak

Journal JoVE
Biologie

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Journal JoVE Biologie

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

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Langerhans İzolasyonu Fare Islet Saflaştırılmış Collagenase ve Nötr Proteaz bir kombinasyonu kullanılarak

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06:59 min

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September 07, 2012

DOI:

DOI:

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06:59 min

September 07, 2012

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Natalie D. Stull, Andrew Breite, Robert McCarthy, Sarah A. Tersey, Raghavendra G. Mirmira^3,4

¹Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, ²VITACYTE, LLC, ³Department of Medicine,Indiana University School of Medicine, ⁴Department of Cellular and Integrative Physiology,Indiana University School of Medicine

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Mouse Islet Of Langerhans Isolation Purified Collagenase Neutral Protease Beta Cell Gene Expression Intracellular Signaling Pathways Secretory Responses Islet Yield Islet Quality Rodent Strain Lot-to-lot Variations Dose Of Enzyme Purified TDEs Rodent Cell Isolations Human Islet Isolations VitaCyte LLC Modified Mouse Islet Isolation Protocol Pancreatic Duct Histopaque Gradient

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