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Mus Islet af Langerhans Isolation ved hjælp af en kombination af renset collagenase og Neutral Protease
Journal JoVE
Biologie
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Journal JoVE Biologie
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

Mus Islet af Langerhans Isolation ved hjælp af en kombination af renset collagenase og Neutral Protease

DOI:
10.3791/4137-v

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06:59 min

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September 07, 2012

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, , , ,
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine,Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology,Indiana University School of Medicine

Chapitres

  • 00:05Titre
  • 01:20Inflation of Pancreas with Collagenase/Protease Mixture
  • 03:58Pancreas Dissociation
  • 05:49Results: Islet Isolation
  • 06:35Conclusion

Summary

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Traduction automatique

En detaljeret beskrivelse af muse ø-isolering er beskrevet anvendelse af teknikken af ​​in situ pancreatisk ductal kanylering og perfusion af en kombination af renset collagenase og neutral protease.

Tags

Mouse Islet Of Langerhans IsolationPurified CollagenaseNeutral ProteaseBeta Cell Gene ExpressionIntracellular Signaling PathwaysSecretory ResponsesIslet YieldIslet QualityRodent StrainLot-to-lot VariationsDose Of EnzymePurified TDEsRodent Cell IsolationsHuman Islet IsolationsVitaCyte LLCModified Mouse Islet Isolation ProtocolPancreatic DuctHistopaque Gradient

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