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小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease

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小鼠胰岛的朗格汉斯的分离纯化的胶原酶和中性蛋白酶结合使用

DOI:

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06:59 min

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September 07, 2012

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Natalie D. Stull, Andrew Breite, Robert McCarthy, Sarah A. Tersey, Raghavendra G. Mirmira^3,4

¹Department of Pediatrics and the Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, ²VITACYTE, LLC, ³Department of Medicine,Indiana University School of Medicine, ⁴Department of Cellular and Integrative Physiology,Indiana University School of Medicine

Chapitres

00:05Titre
01:20Inflation of Pancreas with Collagenase/Protease Mixture
03:58Pancreas Dissociation
05:49Results: Islet Isolation
06:35Conclusion

Summary

Traduction automatique

小鼠胰岛分离的详细描述描述原位胰腺导管插管和灌注纯化的胶原酶和中性蛋白酶的组合中使用的技术。

Tags

Mouse Islet Of Langerhans Isolation Purified Collagenase Neutral Protease Beta Cell Gene Expression Intracellular Signaling Pathways Secretory Responses Islet Yield Islet Quality Rodent Strain Lot-to-lot Variations Dose Of Enzyme Purified TDEs Rodent Cell Isolations Human Islet Isolations VitaCyte LLC Modified Mouse Islet Isolation Protocol Pancreatic Duct Histopaque Gradient

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