/
/
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
12,326 Views
•
09:25 min
•
June 19, 2012
DOI:
Summary
Automatically generated
This article describes a method for the isolation and purification of intact Legionella-containing vacuoles (LCVs) from amoeba and macrophages. The two-step protocol comprises LCV enrichment by immuno-magnetic separation using an antibody against a bacterial LCV marker and further purification by density gradient centrifugation.
Explore More Videos
Pathogen VacuolesLegionella-infected PhagocytesLegionella PneumophilaLegionnaires’ DiseaseLegionella-containing Vacuole (LCV)Icm/Dot Type IV Secretion SystemEffector ProteinsHost ProteinsLipidsOrganellesLCV FormationIntegrative ApproachProteomicsLipidomicsLiquid Chromatography2-D Gel ElectrophoresisMass-spectrometryDictyostelium DiscoideumLeishmaniaListeriaMycobacteriumRhodococcusSalmonella
Read Article
Cite this Article
Hoffmann, C., Finsel, I., Hilbi, H. Purification of Pathogen Vacuoles from Legionella-infected Phagocytes. J. Vis. Exp. (64), e4118, doi:10.3791/4118 (2012).
Copy