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Immunology and Infection

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JoVE Journal Immunology and Infection
Depletion and Reconstitution of Macrophages in Mice
 

Depletion and Reconstitution of Macrophages in Mice

Article DOI: 10.3791/4105-v • 08:50 min • August 1st, 2012
August 1st, 2012 •
1, 2, 3
1Department of Graduate Studies, University of British Columbia, 2Department of Molecular Biology, Vrije Universiteit Amsterdam, 3Department of Pediatrics, University of British Columbia

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résumé
Automatic Translation

Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.

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Depletion Reconstitution Macrophages Mice Innate Immune Response Acquired Immune Response Macrophage Dysfunction Disease Processes Inflammatory Bowel Diseases Polarized Phenotypes Classically Activated Macrophages Alternatively Activated Macrophages IFN-gamma LPS IL-4 IL-13 Inflammation Inflammatory Response Whole Animal Model Depleting Macrophages Complex Environment Phenotypically Defined Polarized Macrophages Bone Marrow Aspirates
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