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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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May 24, 2012
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Summary
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In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.
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Li, M., Husic, N., Lin, Y., Snider, B. J. Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System. J. Vis. Exp. (63), e4031, doi:10.3791/4031 (2012).
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